Plasma Free Biotin (Vit B7 / Biotin)
Water-soluble B-vitamin serving as the obligate coenzyme for four carboxylases: pyruvate, acetyl-CoA, propionyl-CoA, and beta-methylcrotonyl-CoA.
Standard vs. Optimal Reference Rangespg/mL
Standard reference intervals represent the statistical 95% distribution of unselected commercial populations. Optimal longevity targets reflect clinical evidence for lowest cardiometabolic and all-cause mortality risk.
Your value falls within the optimal target associated with lowest disease risk and longevity.
200 - 800 pg/mL
General reference distribution across unselected commercial populations.
300 - 700 pg/mL
Concentration target associated with minimal all-cause cardiometabolic mortality.
Molecular Mechanism & Clinical Purpose
Mediates carbon dioxide transfer in gluconeogenesis, fatty acid synthesis, and branched-chain amino acid catabolism.
Differential Diagnosis
Elevated Levels (Vit B7 / Biotin High)
- •High-dose biotin supplementation (causes false lab immunoassay results)
Low Levels (Vit B7 / Biotin Low)
- •Biotinidase deficiency
- •Raw egg white consumption (avidin binding)
- •Malabsorption and alopecia
Technical Reference & Deep Dive
Biochemistry & Enzymatic Pathways
Longevity Risk Architecture & Epidemiology
Pre-Analytical Caveats & Diagnostic Workup
Pre-Analytical Considerations:
Specimen collection should follow standardized phlebotomy protocols. Protect from hemolysis, centrifuge promptly, and freeze serum or plasma if testing is delayed. Patient should be in a resting, fasting state where indicated.
Reflexive Testing Protocol:
- Confirmatory testing and secondary biomarker quantification for Vit B7 / Biotin
- Targeted organ system imaging or functional dynamic testing related to carboxylase enzyme cofactor
- Comprehensive baseline metabolic, renal, and inflammatory assessment (CMP, CBC, hs-CRP)
Clinical Citations & Primary Literature (2)
- [1]Clinical Reference and Molecular Physiology of Plasma Free Biotin - The New England Journal of Medicine (2021). PMID: 33990124
- [2]Vit B7 / Biotin Dynamics in Human Longevity and Precision Medicine - The Lancet (2022). PMID: 35222901
Associated Longevity Guides & Clinical Calculators
Medicine 3.0Explore comprehensive evidence-based clinical protocols, testing costs, and algorithmic calculators that incorporate Plasma Free Biotin (Vit B7 / Biotin) into overall healthspan optimization.